The Digital Reference World of Academic Librarians.

Throughout the 1990s, the reference departments of academic libraries have seen a rapid evolution from a print-centered world to a digital-intensive one. Online, CD-ROM, and World Wide Web resources are often the first choice of both library users and reference librarians. For the latest measure of how academic libraries incorporate electronic information sources into their reference activities and the effect on library services, a questionnaire was sent to all academic members of the Association of Research Libraries in the 4th quarter of 1997. Over 73% of ARL libraries report more than 100 workstations or terminals. Not surprisingly, the big growth area between 1994 and 1997 is in end-user access to the World Wide Web. Intermediary search services are not dead in academic research libraries, but they are dwindling in numbers, and most services are experiencing sharp declines in the number of searches performed. End-user online searching on commercial search services continues to grow in popularity, beyond the rates predicted at the beginning of this decade - primarily due to OCLC\u27s FirstSearch service

A Decade of Digital Reference: 1991-2001.

Four surveys conducted over a decade provide insights about changes that have occurred in academic library reference services due to new and rapidly evolving technologies. Surveys were sent to the academic members of the Association of Research Libraries four times during the past decade: 1991, 1995, 1997, and 2000. The surveys contained both open-ended questions to gather opinions and factual questions to measure what libraries offer. Libraries adopted digital information sources and services at an increasingly accelerated rate in the 1990s due to the availability of the Internet, in particular the World Wide Web. Digital sources have brought about changes in the physical environment of the reference room, in the type and range of resources available, and in the attitudes and expectations of reference librarians and patrons. The Web is changing what resources are searched, how results are distributed, how instruction is delivered, and relations with faculty. Quality service is still highly valued by reference librarians. The reference librarians surveyed think that as the reference environment has changed, it has helped them to provide better services to patrons

The Impact of Digital Reference on Librarians and library Users

University reference librarians report a variety of effects, both positive and negative, from the rapid and widespread adoption of electronic media during the last decades. Heightened expectations from students and, to a lesser degree, from faculty are noticed by many librarians. Partly because of media hype about the wonders of the Internet and the ubiquity of the World Wide Web, students expect to be able to answer every question and do every research project online. One major impact of electronic services is the growing need for user instruction. Part of the problem is that there are still many students with substandard computer skills. Remote access brings additional instructional challenges. Not surprisingly many librarians report an increase in their workloads as more and different resources are added with no increase in professional staff. Reference interviews take longer, more time is spent with each individual, and librarians have to add more formal instructional sections to their already full days. A few librarians consider that the changes in tasks and focus adversely affect the profession. It was commented that the reference librarian more and more is technical support rather than a resource

Services and Staffing Practices within Academic Libraries Serving College of Osteopathic Medicine Programs: A Mixed Methods Study

This study provides a systematic comparison of services and staffing within academic health sciences libraries serving College of Osteopathic Medicine programs in the U.S. Library directors, not only those affiliated with new medical schools, but also with long- standing, and more traditional schools, can use this data to make a case for new resources, services, or staffing. In addition, medical school administrators may find opportunities in this study’s results for more productive collaborations with the library, in areas such as curriculum-integrated instruction, research development, and scholarly publishing. Finally, leaders within developing medical schools will become better informed about current library services being provided, and staffing trends, in order to plan for an appropriate budget and staffing for their future health sciences libraries

HST/ACS Narrowband Imaging of the Kepler Supernova Remnant

We present narrowband images of the Kepler supernova remnant obtained with the Advanced Camera for Surveys aboard the Hubble Space Telescope. The images, with an angular resolution of 0.05" reveal the structure of the emitting gas in unprecedented detail. Radiative and nonradiative shocks are found in close proximity, unresolvable in gro~md-based spectra, indicating that the pre-shock medium is highly clumped. The ionization structure, traced by differences in the [0 111] to [N 11] flux ratio, varies on subarcsecond scales. The variation is due to 110th differences in shock velocity as well as gradients in the evolutionary stage of the shocks. A pro~llinent complex of knots protruding beyond the boundary of the ren~nallt in the northwest is found to consist of bright radiative knots, collected by arcuate nonradiative filaments. Based on the coincidence of the optical emission with a bright isolated knot of X-ray emission, we infer that this feature is due to a Rayleigh-Taylor finger that formed at the contact discontinuity and overtook the primary blast wave

Molecular evolution of dengue type 2 virus in Thailand

Dengue is a mosquito-borne viral infection that in recent years has become a major international public health concern. Dengue hemorrhagic fever (DHF), first recognized in Southeast Asia in the 1950s, is today a leading cause of childhood death in many countries. The pathogenesis of this illness is poorly understood, mainly because there are no laboratory or animal models of disease. We have studied the genetic relationships of dengue viruses of serotype 2, one of four antigenically distinct dengue virus groups, to determine if viruses obtained from cases of less severe dengue fever (DF) have distinct evolutionary origins from those obtained from DHF cases. A very large number (73) of virus samples from patients with DF or DHF in two locations in Thailand (Bangkok and Kamphaeng Phet) were compared by sequence analysis of 240 nucleotides from the envelope/nonstructural protein 1 (E/NS1) gene junction of the viral genome. Phylogenetic trees generated with these data have been shown to reflect long-term evolutionary relationships among strains. The results suggest that 1) many different virus variants may circulate simultaneously in Thailand, thus reflecting the quasispecies nature of these RNA viruses, in spite of population immunity; 2) viruses belonging to two previously distinct genotypic groups have been isolated from both DF and DHF cases, supporting the view that they arose from a common progenitor and share the potential to cause severe disease; and 3) viruses associated with the potential to cause DHF segregate into what is now one, large genotypic group and they have evolved independently in Southeast Asia for some time

Secreted CLIC3 drives cancer progression through its glutathione-dependent oxidoreductase activity

The secretome of cancer and stromal cells generates a microenvironment that contributes to tumour cell invasion and angiogenesis. Here we compare the secretome of human mammary normal and cancer-associated fibroblasts (CAFs). We discover that the chloride intracellular channel protein 3 (CLIC3) is an abundant component of the CAF secretome. Secreted CLIC3 promotes invasive behaviour of endothelial cells to drive angiogenesis and increases invasiveness of cancer cells both in vivo and in 3D cell culture models, and this requires active transglutaminase-2 (TGM2). CLIC3 acts as a glutathione-dependent oxidoreductase that reduces TGM2 and regulates TGM2 binding to its cofactors. Finally, CLIC3 is also secreted by cancer cells, is abundant in the stromal and tumour compartments of aggressive ovarian cancers and its levels correlate with poor clinical outcome. This work reveals a previously undescribed invasive mechanism whereby the secretion of a glutathione-dependent oxidoreductase drives angiogenesis and cancer progression by promoting TGM2-dependent invasion

Assessment of the cortisol awakening response: Real-time analysis and curvilinear effects of sample timing inaccuracy

The cortisol awakening response (CAR) is typically measured in the domestic setting. Moderate sample timing inaccuracy has been shown to result in erroneous CAR estimates and such inaccuracy has been shown partially to explain inconsistency in the CAR literature. The need for more reliable measurement of the CAR has recently been highlighted in expert consensus guidelines where it was pointed out that less than 6% of published studies provided electronic-monitoring of saliva sampling time in the post-awakening period. Analyses of a merged data-set of published studies from our laboratory are presented. To qualify for selection, both time of awakening and collection of the first sample must have been verified by electronic-monitoring and sampling commenced within 15 min of awakening. Participants (n = 128) were young (median age of 20 years) and healthy. Cortisol values were determined in the 45 min post-awakening period on 215 sampling days. On 127 days, delay between verified awakening and collection of the first sample was less than 3 min (‘no delay’ group); on 45 days there was a delay of 4–6 min (‘short delay’ group); on 43 days the delay was 7–15 min (‘moderate delay’ group). Cortisol values for verified sampling times accurately mapped on to the typical post-awakening cortisol growth curve, regardless of whether sampling deviated from desired protocol timings. This provides support for incorporating rather than excluding delayed data (up to 15 min) in CAR analyses. For this population the fitted cortisol growth curve equation predicted a mean cortisol awakening level of 6 nmols/l (±1 for 95% CI) and a mean CAR rise of 6 nmols/l (±2 for 95% CI). We also modelled the relationship between real delay and CAR magnitude, when the CAR is calculated erroneously by incorrectly assuming adherence to protocol time. Findings supported a curvilinear hypothesis in relation to effects of sample delay on the CAR. Short delays of 4–6 min between awakening and commencement of saliva sampling resulted an overestimated CAR. Moderate delays of 7–15 min were associated with an underestimated CAR. Findings emphasize the need to employ electronic-monitoring of sampling accuracy when measuring the CAR in the domestic setting

The Role of Neutrophils during Mild and Severe Influenza Virus Infections of Mice

Neutrophils have been implicated in both protective and pathological responses following influenza virus infections. We have used mAb 1A8 (anti-Ly6G) to specifically deplete LyG6high neutrophils and induce neutropenia in mice infected with virus strains known to differ in virulence. Mice were also treated with mAb RB6-8C5 (anti-Ly6C/G or anti-Gr-1), a mAb widely used to investigate the role of neutrophils in mice that has been shown to bind and deplete additional leukocyte subsets. Using mAb 1A8, we confirm the beneficial role of neutrophils in mice infected with virus strains of intermediate (HKx31; H3N2) or high (PR8; H1N1) virulence whereas treatment of mice infected with an avirulent strain (BJx109; H3N2) did not affect disease or virus replication. Treatment of BJx109-infected mice with mAb RB6-8C5 was, however, associated with significant weight loss and enhanced virus replication indicating that other Gr-1+ cells, not neutrophils, limit disease severity during mild influenza infections

The driver mutational landscape of ovarian squamous cell carcinomas arising in mature cystic teratoma

Purpose. We sought to identify the genomic abnormalities in squamous cell carcinomas (SCC) arising in ovarian mature cystic teratoma (MCT), a rare gynaecological malignancy of poor prognosis. Experimental design. We performed copy number, mutational state and zygosity analysis of 151 genes in SCC arising in MCT (n=25) using next-generation sequencing. The presence of high/intermediate risk HPV genotypes was assessed by quantitative PCR. Genomic events were correlated with clinical features and outcome Results. MCT had a low mutation burden with a mean of only 1 mutation per case. Zygosity analyses of MCT indicated four separate patterns, suggesting that MCT can arise from errors at various stages of oogenesis. A total of 244 abnormalities were identified in 79 genes in MCT-associated SCC, and the overall mutational burden was high (mean 10.2 mutations per megabase). No SCC was positive for HPV. The most frequently altered genes in SCC were TP53 (20/25 cases, 80%), PIK3CA (13/25 cases, 52%) and CDKN2A (11/25 cases, 44%). Mutation in TP53 was associated with improved overall survival. In 8/20 cases with TP53 mutations, two or more variants were identified, which were bi-allelic. Conclusions. Ovarian SCC arising in MCT has a high mutational burden with TP53 mutation the most common abnormality. The presence TP53 mutation is a good prognostic factor. SCC arising in MCT share similar mutation profiles to other SCC. Given their rarity, they should be included in basket studies that recruit patients with SCC of other organs